A Novel Corynebacterium glutamicum l -Glutamate Exporter
نویسندگان
چکیده
منابع مشابه
Ethambutol, a cell wall inhibitor of Mycobacterium tuberculosis, elicits L-glutamate efflux of Corynebacterium glutamicum.
Corynebacterium glutamicum is used for the large-scale production of L-glutamate, but the efflux of this amino acid is poorly understood. This study shows that addition of ethambutol (EMB) to growing cultures of C. glutamicum causes L-glutamate efflux at rates of up to 15 nmol min(-1) (mg dry wt)(-1), whereas in the absence of EMB, no efflux occurs. EMB is used for the treatment of Mycobacteriu...
متن کاملReducing lactate secretion by ldhA Deletion in L-glutamate- producing strain Corynebacterium glutamicum GDK-9
L-lactate is one of main byproducts excreted in to the fermentation medium. To improve L-glutamate production and reduce L-lactate accumulation, L-lactate dehydrogenase-encoding gene ldhA was knocked out from L-glutamate producing strain Corynebacterium glutamicum GDK-9, designated GDK-9ΔldhA. GDK-9ΔldhA produced approximately 10.1% more L-glutamate than the GDK-9, and yielded lower levels of s...
متن کاملL-glutamate efflux with Corynebacterium glutamicum: why is penicillin treatment or Tween addition doing the same?
Corynebacterium glutamicum is known for its ability to massively excrete cellularly synthesized L-glutamate. Together with Mycobacteria and Nocardia this bacterium belongs to the CMN group of the Gram-positive eubacteria. The capacity of C. glutamicum to excrete glutamate has been used for more than 40 years to produce this amino acid in ever increasing quantities of currently about 10 6 tonnes...
متن کاملL-Glutamate production by lysozyme-sensitive Corynebacterium glutamicum ltsA mutant strains
BACKGROUND A non-pathogenic species of coryneform bacteria, Corynebacterium glutamicum, was originally isolated as an L-glutamate producing bacterium and is now used for fermentative production of various amino acids. A mutation in the C. glutamicum ltsA gene caused susceptibility to lysozyme, temperature-sensitive growth, and L-glutamate production. RESULTS The characteristics of eight lysoz...
متن کاملAltered metabolic flux due to deletion of odhA causes L-glutamate overproduction in Corynebacterium glutamicum.
L-glutamate overproduction in Corynebacterium glutamicum, a biotin auxotroph, is induced by biotin limitation or by treatment with certain fatty acid ester surfactants or with penicillin. We have analyzed the relationship between the inductions, 2-oxoglutarate dehydrogenase complex (ODHC) activity, and L-glutamate production. Here we show that a strain deleted for odhA and completely lacking OD...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2018
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02691-17